Open AccessCloning and characterization of the phenylalanyl‐tRNA synthetase β subunit gene from Candida albicans
Author(s) -
Marcilla Antonio,
Pallotti Claudia,
GomezLobo Maria,
Caballero Pedro,
Valentin Eulogio,
Sentandreu Rafael
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12946.x
Subject(s) - candida albicans , biology , open reading frame , cloning (programming) , gene , transfer rna , microbiology and biotechnology , saccharomyces cerevisiae , protein subunit , clone (java method) , biochemistry , genetics , peptide sequence , rna , computer science , programming language
Abstract A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4‐kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl‐tRNA synthetase β subunit (FRS1) of Saccharomyces cerevisiae . We named it CaFRS1 . It is located in a single copy in chromosome R, Sfi I fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans , suggesting its requirement thereof in initial steps of the cell wall synthesis.