Open AccessSporulation and time course expression of sigma‐factor homologous genes in Clostridium acetobutylicum
Author(s) -
Santangelo Joseph D,
Kuhn Anita,
TreunerLange Anke,
Dürre Peter
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12943.x
Subject(s) - clostridium acetobutylicum , sigma factor , operon , bacillus subtilis , gene , biology , homology (biology) , clostridium , microbiology and biotechnology , genetics , endospore , transcription (linguistics) , promoter , spore , gene expression , bacteria , biochemistry , escherichia coli , butanol , linguistics , philosophy , ethanol
The gene for the vegetative sigma factor A of Clostridium acetobutylicum was constitutively transcribed during growth and formed an operon together with dnaE . Sporulation‐specific sigma factors E, G, and K were sequentially induced shortly before mature endospores could be detected. Maximal transcription in the course of spore formation was found to be in the order sigE‐sigG‐sigK , thus matching the pattern described for Bacillus subtilis . From primer extension experiments promoter structures could be deduced with high homology to the Bacillus consensus. Upstream of the spoIIGA‐sigE operon a gene with significant similarity to ftsZ could be detected.