Open AccessPhysiology of exolaccase production by Thelephora terrestris
Author(s) -
Kanunfre Carla C,
Zancan Glaci T
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12942.x
Subject(s) - guaiacol , laccase , abts , biochemistry , extracellular , inducer , chemistry , ammonium , food science , biomass (ecology) , biology , enzyme , organic chemistry , ecology , dpph , gene , antioxidant
Thelephora terrestris , an ectomycorrhizal hymenomycete, produces extracellular laccase when grown in minimal liquid medium. The enzyme was characterized as a protein of 66 kDa. The optimal pH varied depending on the substrate utilized, being 5.0 for syringaldazine, 4.8 for guaiacol and 3.4 for ABTS. The K m was 2.52±0.4 μM for syringaldazine, 16±1.9 μM for ABTS and 120.6±4.9 μM for guaiacol. The secretion of laccase was biomass‐dependent and independent of the glucose concentration in the medium. The addition of inducers did not increase laccase production which was dependent upon the ammonium phosphate concentration.