Open AccessGrowth phase‐dependent active transport of pyridoxine in a fission yeast, Schizosaccharomyces pombe
Author(s) -
Yagi Toshiharu,
Tanouchi Atsuyo,
Hiraoka Yasuhisa
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12941.x
Subject(s) - schizosaccharomyces pombe , pyridoxine , yeast , sodium azide , thiamine , biochemistry , chemistry , dinitrophenol , azide , amiloride , sodium , saccharomyces cerevisiae , organic chemistry
Schizosaccharomyces pombe showed maximum pyridoxine uptake activity around 10 h after starting cultivation. High concentrations of thiamine and pyridoxine in the medium did not affect the activity or the time but changed intracellular levels of vitamin B 6 compounds. Pyridoxine was taken up by a saturable mechanism with two kinds of affinity ( K m 22.4 μM and 118 μM). The uptake depended on the energy produced anaerobically with an optimum pH of 4.5. The uptake was completely inhibited by amiloride, sodium azide or 2,4‐dinitrophenol. The uptake system of the fission yeast was different in various respects from that of a budding yeast.