Open AccessGeneral transducing phages like Salmonella phage P22 isolated using a smooth strain of Escherichia coli as host
Author(s) -
Dhillon Tarlochan S,
Poon Alice P.W,
Chan Dorothy,
Clark Alvin J
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12938.x
Subject(s) - escherichia coli , microbiology and biotechnology , salmonella enterica , biology , strain (injury) , auxotrophy , salmonella , bacteriophage , mutant , temperateness , bacteria , lysogenic cycle , genetics , gene , anatomy
A smooth colony strain, resistant to phages λ and P22, was isolated from sewage and identified as Escherichia coli (strain H). Four temperate phages plaquing on strain H were isolated from sewage. The archetype, HK620, does not plaque on strains C and K12 of E. coli nor on the LT2 strain of Salmonella enterica. Bacterial mutants resistant to a clear plaque mutant of HK620 produce rough colonies. Some are also galactose‐negative, a few are histidine auxotrophs, and most show sensitivity to λ. HK620 can transduce a wide variety of auxotrophic mutants of E. coli H to prototrophy. It can recombine with λ but its virions resemble those of P22.