Open AccessGenomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP‐PCR and restriction fragment end‐labelling
Author(s) -
Doorn Nathalie E.M,
Namavar Ferry,
Kusters Johannes G,
Rees Emmelien P,
Kuipers Ernst J,
Graaff Johannes
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12904.x
Subject(s) - biology , typing , helicobacter pylori , polymerase chain reaction , restriction fragment length polymorphism , genomic dna , dna profiling , genetics , helicobacter , restriction fragment , dna , microbiology and biotechnology , gene
Genetic diversity of 32 Helicobacter pylori strains isolated from patients with gastritis, gastric or duodenal ulcer, carcinoma, or lymphoma was determined by repetitive sequence element polymerase chain reaction (REP‐PCR), and by the new typing method restriction fragment end‐labelling (RFEL). Furthermore, these two methods were used to investigate a possible correlation between clinical symptoms and the genetic background of Helicobacter pylori . Both REP‐PCR and RFEL revealed 31 different patterns for the 32 strains tested, but the pair of isolates with identical REP‐PCR patterns was not the same as the pair of isolates with identical RFEL patterns. Computer‐assisted analysis of the DNA fingerprints was used to determine similarity coefficients. This analysis revealed no clustering of disease‐specific strains by any of the two methods.