Open AccessVariability in the distribution and composition of insertion sequence‐like elements in strains of Mycoplasma fermentans
Author(s) -
Pitcher David,
Hilbocus Jhassen
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12898.x
Subject(s) - biology , insertion sequence , polymerase chain reaction , genetics , restriction enzyme , mollicutes , strain (injury) , genomic dna , genome , dna , sequence (biology) , mycoplasma , restriction fragment length polymorphism , repeated sequence , sequence analysis , microbiology and biotechnology , transposable element , gene , anatomy
Mycoplasma fermentans has been reported to be pathogenic for man. All fourteen strains tested contain an insertion sequence‐like element (ISLE) which may be present in multiple copies. To determine whether ISLE copies are similarly distributed in different strains of M. fermentans , restriction enzyme digest fragments of genomic DNA from 14 isolates, from a variety of sources, were separated by electrophoresis, blotted and hybridized to a biotin labelled polymerase chain reaction (PCR) amplified fragment of ISLE. A range of patterns was observed suggesting that the element has a tendency to undergo rearrangement within the genome. Analysis of ISLE sequences revealed inter‐ and intra‐strain polymorphisms.