Open AccessHuman transferrin receptor is active and plasma membrane‐targeted in yeast
Author(s) -
Terng HarnJing,
Geßner Reinhard,
Fuchs Hendrik,
Stahl Ulf,
Lang Christine
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12891.x
Subject(s) - transferrin , yeast , transferrin receptor , saccharomyces cerevisiae , biochemistry , receptor , biology , glycosylation , microbiology and biotechnology
The human transferrin receptor, a type II plasma membrane protein which mediates iron transport in human cells, was expressed in the yeast Saccharomyces cerevisiae . The transferrin receptor synthesized by yeast cells was posttranslationally modified comparable to the native receptor with respect to glycosylation and dimer formation. The location of the expressed receptor in the yeast plasma membrane indicates that the targeting of this type II membrane protein shares similar mechanisms in yeast and mammalian cells. The yeast‐expressed transferrin receptor showed binding activity towards its natural ligand, transferrin in an ELISA binding assay.