Open AccessIncrease of sulfite tolerance in Oenococcus oeni by means of acidic adaptation
Author(s) -
Guzzo Jean,
Jobin MichelPhilippe,
Diviès Charles
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12888.x
Subject(s) - oenococcus oeni , sulfite , malolactic fermentation , sulfite oxidase , winemaking , chemistry , biochemistry , wine , bacteria , fermentation , food science , biology , lactic acid , genetics
Sulfite is an antimicrobial agent used at the beginning of winemaking to avoid development of undesirable microorganisms. However, Oenococcus oeni , which is mainly responsible for the malolactic fermentation, has to grow in wine and therefore has to be resistant to sulfite. This study showed that acid‐adapted cells of O. oeni survived better than non‐adapted cells in the presence of a high sulfite concentration (30 mg l −1 ). Addition of a sub‐lethal concentration of sulfite (15 mg l −1 ) during the adaptation step in acidic medium increases the sulfite tolerance. Moreover, sulfite appeared to be able to induce a heat shock‐like response. Our results suggest that pH homeostasis mechanisms and stress protein synthesis could be involved in the induction of sulfite tolerance in O. oeni .