Open AccessAnalysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase ( ndk ) and folylpolyglutamate synthetase ( folC ) genes
Author(s) -
Burger Annette,
Brandt Birgit,
Süsstrunk Urs,
Thompson Charles J,
Wohlleben Wolfgang
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12873.x
Subject(s) - streptomyces coelicolor , nucleoside diphosphate kinase , gene , nucleoside , locus (genetics) , biochemistry , chemistry , biology , genetics , mutant
A 3.6‐kb DNA fragment from Streptomyces coelicolor A3(2) with the genes valS probably encoding a valyl‐tRNA synthetase, folC encoding folylpolyglutamate synthetase, and ndk encoding a nucleoside diphosphate kinase was analysed. folC and ndk are separated by a small open reading frame of unknown function, orfX . The deduced folC gene product is a protein of 46 677 Da whose sequence is similar to other folylpolyglutamate synthetases and folylpolyglutamate synthetase‐dihydrofolate synthetases from both Gram‐positive and Gram‐negative bacteria. After cloning folC behind the lacZ promoter, the Streptomyces folC complemented a folC mutant of Escherichia coli . An essential function for Streptomyces folC was suggested by the fact that it could not be mutated using a conventional gene disruption technique.