Open AccessMacrorestriction analysis of Desulfurella acetivorans and Desulfurella multipotens
Author(s) -
Pradella Silke,
Hippe Hans,
Stackebrandt Erko
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12852.x
Subject(s) - extrachromosomal dna , biology , genetics , restriction enzyme , genome , pulsed field gel electrophoresis , endonuclease , genomic dna , chromosome , microbiology and biotechnology , dna , gene , genotype
The genomes of the phylogenetically and physiologically unique bacteria Desulfurella acetivorans DSM 5264 T and D. multipotens DSM 8415 T were characterized and compared by pulsed field gel electrophoresis (PFGE). Macrorestriction patterns made of large PFGE separated DNA fragments were generated by digesting the genomic DNAs of both strains with the rare cutting restriction endonucleases Apa I, Asc I, Eag I, Rsr II, Sac II, Sal I as well as with the intron encoded endonuclease I‐ Ceu I. The sum of calculated fragment sizes from digests of the first six enzymes yielded estimates for the chromosome sizes of D. acetivorans with a mean of 1939.0±26.0 kb and for D. multipotens with a mean of 1864.0±23.0 kb. Within the patterns obtained from Eag I and Rsr II cleavages the apparent differences could be attributed to DNA insertion or deletion and to point mutation. The single, circular chromosomes of the two strains contain two copies of 23S rRNA genes each. Different extrachromosomal elements were detected in both strains.