Open AccessPolymorphisms of the genes encoding cruzipain, the major cysteine proteinase of Trypanosoma cruzi , in the region encoding the C‐terminal domain
Author(s) -
Martıćnez Javier,
Henriksson Jan,
Ridåker Maria,
Pettersson Ulf,
Cazzulo Juan José
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12838.x
Subject(s) - trypanosoma cruzi , complementary dna , biology , gene , amino acid , peptide sequence , cysteine , biochemistry , glycosylation , microbiology and biotechnology , genetics , enzyme , parasite hosting , world wide web , computer science
Forty‐eight cDNA clones obtained from different developmental stages of Trypanosoma cruzi and all encoding the C‐terminal domain of the major cysteine proteinase (cruzipain) have been sequenced. A number of polymorphisms were detected, seven of them resulting in amino acid replacements. The predicted pI values of the corresponding gene products varied between 7.05 and 8.12. These changes in amino acid sequence, together with previously reported variations in carbohydrate composition at the only N‐glycosylation site in the C‐terminal domain, may account for most of the heterogeneities found in the mature enzyme.