Open AccessPutative protein serine/threonine kinase genes are located in several positions on the chromosome of Streptomyces coelicolor A3(2)
Author(s) -
Hirakata Toshiyuki,
Kieser Helen,
Hopwood David,
Urabe Hiroaki,
Ogarawa Hiroshi
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12833.x
Subject(s) - streptomyces coelicolor , gene , threonine , c raf , microbiology and biotechnology , casein kinase 2 , biology , protein kinase a , serine , nucleic acid sequence , genetics , kinase , biochemistry , cyclin dependent kinase 2 , phosphorylation , mutant
Seven DNA fragments containing putative protein serine/threonine kinase genes were cloned from Streptomyces coelicolor A3(2) by hybridization with a 165‐bp amplified polymerase chain reaction product of pkaB containing kinase subdomains VI, VII and VIII. Among them, the nucleotide sequences of three fragments containing subdomains VI, VII and VIII were identical, while those of one fragment were identical with that of afsK , another protein serine/threonine kinase gene. However, these kinase‐like genes with very similar nucleotide sequences were distributed on the chromosome of S. coelicolor A3(2).