Open AccessAmino acid transport in Lactobacillus helveticus
Author(s) -
Nakajima Hadjime,
Kunji Edmund R.S,
Poolman Bert,
Konings Wil N
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12828.x
Subject(s) - lactobacillus helveticus , valine , amino acid , biochemistry , phenylalanine , leucine , lactococcus lactis , isoleucine , chemistry , tryptophan , threonine , amino acid synthesis , lysine , biology , lactobacillus , lactic acid , bacteria , serine , phosphorylation , fermentation , genetics
Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus NCDO2712 actively transported and accumulated the essential and growth stimulating amino acids (leucine, isoleucine, valine, threonine, lysine, aspartic acid, glutamic acid, tyrosine, arginine, and histidine). Uptake of proline, phenylalanine and tryptophan was not observed. Transport studies in isolated membrane vesicles of L. helveticus fused with liposomes to reduce the passive proton permeability revealed that amino acids leucine, isoleucine, valine, threonine, and lysine are transported by a proton motive force coupled system. These results indicated that L. helveticus and Lactococcus lactis amino acid transport systems are similar.