Open AccessZ ‐phenylacetaldoxime degradation by a novel aldoxime dehydratase from Bacillus sp. strain OxB‐1
Author(s) -
Asano Yasuhisa,
Kato Yasuo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12818.x
Subject(s) - dehydratase , phenylacetate , nitrilase , nitrile hydratase , strain (injury) , enzyme , bacteria , rhodococcus , chemistry , nitrile , brevibacterium , amidase , degradation (telecommunications) , biochemistry , organic chemistry , biology , telecommunications , computer science , anatomy , microorganism , genetics
Z ‐phenylacetaldoxime ( Z ‐PAOx) degrading bacterium, identified as Bacillus sp. strain OxB‐1, was isolated from soil after 2 months acclimation. The enzyme involved in the degradation of Z ‐PAOx was induced by the aldoxime and required FMN for its activity. The enzyme was partially purified from the cell‐free extract of the strain and shown to catalyze the stoichiometric dehydration reaction of Z ‐PAOx to form phenylacetonitrile (PAN). Activities of nitrilase and amidase acting on PAN and phenylacetamide (PAAm), respectively, to form phenylacetate (PAA) were found in the strain grown on Z ‐PAOx. This is the first report of aldoxime dehydratase co‐existing with nitrile degrading enzymes in bacteria.