Open AccessTermitomyces clypeatus controls secretion of extracellular amyloglucosidase by regulating exocytosis of vacuolar enzyme
Author(s) -
Sengupta Trishna,
Hazra P.P,
Mukhopadhyay A,
Sengupta S
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12807.x
Subject(s) - exocytosis , extracellular , amylase , vacuole , sucrase , biochemistry , enzyme , intracellular , biology , chemistry , secretion , cytoplasm
An extracellular amyloglucosidase (56 kDa) of Termitomyces clypeatus which was accumulated intracellularly in absence of Krebs cycle acids in dextrin medium, remained mostly inside the plasma membrane. The enzyme was localised in mycelial vacuoles as a sucrase‐amyloglucosidase aggregate. The intracellular pool contained both the free sucrase and the aggregate, while vacuoles contained only aggregate without any free enzyme. The aggregate, partially purified to PAGE homogeneity, contained amyloglucosidase and sucrase in the ratio of 1:1. A cellular regulation, influenced by the presence of Krebs cycle acids, was indicated to be present at the level of exocytosis of vacuolar enzyme.