Open AccessTransformation of the entomopathogenic fungus, Metarhizium flavoviride strain CG423 to benomyl resistance
Author(s) -
ValadaresInglis M.Cléria,
Inglis Peter W.
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb13878.x
Subject(s) - benomyl , entomopathogenic fungus , neurospora crassa , biology , transformation (genetics) , mutant , microbiology and biotechnology , gene , botany , genetics , fungicide , biological pest control , beauveria bassiana
Metarhizium flavoviride strain CG423 is being developed as a mycoinsecticide against grasshoppers. This strain has been transformed to resistance to the fungicide benomyl by a polyethylene glycol (PEG)‐mediated procedure using a mutant tubulin gene from Neurospora crassa . Transformation frequencies of up to 84 transformants per microgram of transforming DNA were achieved. Benomyl‐resistant transformants were obtained that could tolerate greater than 30 μg ml −1 benomyl. Southern blot analysis of genomic DNA reveals that the mechanism of genetic transformation of all transformants was by homologous gene replacement of the β‐tubulin allele.