Open AccessNickel resistance in Escherichia coli V38 is dependent on the concentration used for induction
Author(s) -
Rubikas Jonas,
Matulis Daumantas,
Leipus Arunas,
Urbaitiene Daiva
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb13877.x
Subject(s) - efflux , incubation , escherichia coli , nickel , bacteria , microbiology and biotechnology , incubation period , chemistry , strain (injury) , metabolism , biology , biochemistry , nuclear chemistry , chromatography , genetics , organic chemistry , anatomy , gene
Strain Escherichia coli V38 resistant to 4 mM NiCl 2 was isolated from the city sewage sludge. It showed low nickel accumulation by cells and nickel ion efflux. Cells were pregrown (induced) overnight in the presence of Ni 2+ , then the culture was kept on ice for 20–30 min and transferred to 37°C for further incubation. When the Ni 2+ concentration during growth was the same as during incubation, there was no noticeable accumulation of Ni 2+ . When the Ni 2+ concentration during incubation was higher than that used for induction, uptake of 63 Ni 2+ and delayed efflux were seen. The uptake and delay of both efflux and growth were directly proportional to the difference between the concentrations used for induction and incubation. Active nickel ion uptake was seen in cells taken from cultures in the delayed efflux period.