Open AccessUse of SSU rDNA group‐I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena 1
Author(s) -
Fulton Ciaran E,
Brown Averil E
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12790.x
Subject(s) - monilinia fructicola , biology , intron , primer (cosmetics) , pathogen , microbiology and biotechnology , gene , botany , genetics , postharvest , chemistry , organic chemistry
Monilinia fructicola , M. laxa and M. fructigena are the causal agents of brown rot of pome and stone fruits. M. fructicola is not present in Europe and is classed as a quarantine pathogen in EU countries. A 418‐bp group‐I intron has been located in the small subunit (SSU) rDNA gene of M. fructicola which is absent from M. laxa and M. fructigena . PCR primers specific to the 3′‐region of the intron together with the SSU rDNA primer NS5 were able to amplify a 444‐bp product from M. fructicola and fruit tissue infected with M. fructicola but not from the other two species. This allows for the rapid and sensitive detection of this pathogen in planta.