Open AccessCloning of a cDNA fragment encoding part of the protein moiety of the 58‐kDa fibrinogen‐binding mannoprotein of Candida albicans
Author(s) -
LópezRibot José L,
Sepúlveda Pilar,
Cervera Ana M,
Roig Patricia,
Gozalbo Daniel,
Martıćnez José P
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12784.x
Subject(s) - immunoscreening , candida albicans , complementary dna , open reading frame , biology , gene , cdna library , microbiology and biotechnology , nucleic acid sequence , biochemistry , peptide sequence , molecular cloning
Immunoscreening of a Candida albicans expression library with antibodies against the 58 kDa fibrinogen‐binding mannoprotein (mp58) of the fungus resulted in the isolation of clones encoding the protein moiety of this molecule. Sequence of the 0.9 kb cDNA of one of the clones selected for further analysis, revealed an open reading frame coding for 292 amino acids, which displays sequence similarity to proteins belonging to a family of immunodominant antigens of Aspergillus spp. The gene corresponding to this cDNA was named FBP1 (fibrinogen‐binding protein). These results represent the first report on the identification of C. albicans genes encoding surface receptors for host proteins.