Open AccessTelomeric DNA of Botrytis cinerea : a useful tool for strain identification
Author(s) -
Levis Caroline,
Giraud Tatiana,
Dutertre Murielle,
Fortini Dominique,
Brygoo Yves
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12783.x
Subject(s) - botrytis cinerea , biology , telomere , restriction fragment length polymorphism , southern blot , fungus , primer (cosmetics) , amplified fragment length polymorphism , strain (injury) , microbiology and biotechnology , dna , polymerase chain reaction , genetics , botany , gene , genetic diversity , population , chemistry , demography , organic chemistry , anatomy , sociology
Telomeric DNA was isolated from the phytopathogenic fungus Botrytis cinerea by PCR using only the oligonucleotide primer (CCCTAA) 4 . As with other filamentous fungi, B. cinerea has a short TTAGGG telomeric repeat. Telomere‐linked restriction fragment length polymorphism (RFLP) was found in strains of B. cinerea isolated from different host plants collected from different regions at different periods. Almost every strain had a specific RFLP pattern, including those collected from the same plant one month apart. Thus, this marker appears to be an excellent tool to show the great polymorphism of B. cinerea strains by fingerprinting. The Southern blots of some strains of B. cinerea showed one band which was much more intense than the others, suggesting that the majority of telomere‐associated sequences have the same sequence.