Open AccessListeria monocytogenes ‐infected human umbilical vein endothelial cells: internalin‐independent invasion, intracellular growth, movement, and host cell responses
Author(s) -
Greiffenberg Lars,
Sokolovic Zeljka,
Schnittler HansJoachim,
Spory Andrea,
Böckmann Regine,
Goebel Werner,
Kuhn Michael
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12768.x
Subject(s) - umbilical vein , listeria monocytogenes , human umbilical vein endothelial cell , biology , microbiology and biotechnology , intracellular , endothelial stem cell , immunology , bacteria , in vitro , biochemistry , genetics
The interaction of Listeria monocytogenes with human umbilical vein endothelial cells was studied. We show that L. monocytogenes invades human umbilical vein endothelial cells independently of internalin A, internalin B, internalin C, and ActA. L. monocytogenes replicates efficiently inside the cells and moves intracellularly by the induction of actin polymerization. We further show that L. monocytogenes ‐infection of human umbilical vein endothelial cells induces interleukin‐6 and interleukin‐8 expression during the first 6 h of infection. The expression of MCP‐1 and the adhesion molecules VCAM‐1 and ICAM‐1 was not altered under the experimental conditions used here.