Open AccessCloning and characterization of the lytB gene of Campylobacter jejuni
Author(s) -
Wösten Marc M.S.M,
Ishiguro Ed E,
Zeijst Bernard A.M
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12761.x
Subject(s) - campylobacter jejuni , biology , gene , mutant , escherichia coli , cloning (programming) , microbiology and biotechnology , genetics , ribosomal protein , molecular cloning , bacteria , peptide sequence , rna , ribosome , computer science , programming language
LytB of Escherichia coli is an essential gene involved in penicillin tolerance and the stringent response. The lytB gene of Campylobacter jejuni was cloned and characterized. It could complement a temperature‐sensitive E. coli lytB mutant. The C. jejuni lytB gene encodes a protein of 277 amino acids that has 34, 36 and 40% amino acid identity with the LytB proteins of E. coli , Haemophilus influenzae , and Synechocystis sp. PCC6803, respectively. The lytB gene is situated between the aroA gene and a gene that encodes ribosomal protein S1.