Open AccessDevelopment of a polymerase chain reaction assay for Mycoplasma salivarium by using the nucleotide sequence within aminopeptidase My gene
Author(s) -
Yoshikawa Tetsuya,
Shibata Kenichiro,
Kaga Masayuki,
Sato Yoshiaki,
Oguchi Haruhisa,
Totsuka Yasunori,
Watanabe Tsuguo
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12741.x
Subject(s) - polymerase chain reaction , microbiology and biotechnology , nucleic acid sequence , biology , mollicutes , gene , aminopeptidase , dna , inverse polymerase chain reaction , mycoplasma , biochemistry , genetics , multiplex polymerase chain reaction , amino acid , leucine
A polymerase chain reaction assay for a 278‐nucleotide DNA fragment within aminopeptidase My gene of Mycoplasma salivarium was developed. The assay amplified M. salivarium DNA, but did not amplify DNAs of other mollicutes, bacteria and mammalian cells. The detection limit of the assay was 10 fg of DNA, approximately equivalent to 10 organisms.