Open AccessPlasmids from Halomonas eurihalina , a microorganism which produces an exopolysaccharide of biotechnological interest
Author(s) -
Llamas Inmaculada,
Moral Ana,
Béjar Victoria,
Girón Marıća Dolores,
Salto Rafael,
Quesada Emilia
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12736.x
Subject(s) - plasmid , halomonas , halophile , biology , microbiology and biotechnology , bacteria , cloning vector , cloning (programming) , homology (biology) , genetics , molecular cloning , gene , gene expression , computer science , programming language
The moderately halophilic bacterium Halomonas eurihalina strain F2‐7, able to produce an exopolysaccharide, was found to contain two plasmids named pVE1 and pVE2, of 8.1 and 5.8 kb respectively. We found no evidence for the involvement of these plasmids in the expression of the mucoid phenotype. Restriction maps of both plasmids were constructed. Southern hybridization revealed similarities between them but excluded the existence of sequences homologous to other plasmids isolated from the Halomonas species. Neither pVE1 nor pVE2 displayed any homology with other plasmids isolated from moderate halophiles. The occurrence of similar plasmids in other strains of Halomonas eurihalina , isolated from hypersaline soils, has been detected. These small plasmids may be useful for the development of cloning vectors for moderately halophilic bacteria.