Open AccessIdentification of restriction barriers in Pasteurella multocida
Author(s) -
Hoskins Isobel C,
Lax Alistair J
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12731.x
Subject(s) - psti , pasteurella multocida , plasmid , biology , microbiology and biotechnology , escherichia coli , dna , bacteria , genetics , gene
Several naturally occurring antibiotic resistance plasmids were isolated from Pasteurella multocida type D strains. One plasmid, pPM1, was used to study transfer of DNA among P. multocida strains, and could be transferred into Escherichia coli and some P. multocida isolates. However, pPM1 could only be transferred into the toxigenic P. multocida LFB3 at very low frequency. Plasmid recovered from the electrotransformants could be transferred to LFB3 at high frequency. These plasmid DNAs were resistant to Pst I, and sensitive to Dpn I digestion. Sensitivity to Dpn I was common to all the P. multocida DNAs, but resistance to Pst I was confined to LFB3. Plasmid pPM1 treated with Pst I methylase was able to transform LFB3 at an increased frequency compared to unmethylated DNA, suggesting that LFB3 has a restriction system which cleaves at or near Pst I sites.