Open AccessAnalysis of the G93E mutant allele of KpsM, the membrane component of an ABC transporter involved in polysialic acid translocation in Escherichia coli K1
Author(s) -
Pigeon Ronald P,
Silver Richard P
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12730.x
Subject(s) - polysialic acid , chromosomal translocation , escherichia coli , mutant , allele , biology , microbiology and biotechnology , wild type , mutation , chemistry , biochemistry , gene , cell , cell adhesion , neural cell adhesion molecule
KpsM is an integral membrane protein involved in the translocation of the polysialic acid capsule of Escherichia coli K1. The kpsMG93E allele is a point mutation in the first cytoplasmic loop (CI) of KpsM which partially disrupts translocation of the capsule. While producing polymer of wild‐type length, strains harboring the G93E allele exhibit a decreased production of capsular polymer and a reduced rate of polymer translocation to the cell surface.