Open AccessRFLP‐PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas
Author(s) -
Cascón Soriano Alberto,
Anguita Castillo Juan,
Hernanz Moral Carmen,
Sánchez Salazar Marıća,
Yugueros Marcos Javier,
Naharro Carrasco Germán
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12727.x
Subject(s) - aroa , biology , aeromonas salmonicida , restriction fragment length polymorphism , gene , aeromonas , polymerase chain reaction , nucleic acid sequence , genetics , sequence analysis , oligonucleotide , hybridization probe , microbiology and biotechnology , bacteria , enterobacteriaceae , escherichia coli
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3‐phosphoshikimate‐1‐carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. A 1236‐bp DNA fragment, representing most of the aroA gene, according to the nucleotide sequence of A. salmonicida , was amplified from all Aeromonas species tested, which represented most of the 14 hybridization groups. Hae II digestion of the 1236‐bp fragment generated a restriction fragment length polymorphisms which could be used as a powerful tool for identification of aeromonads to the genus level.