Open AccessHigh frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus
Author(s) -
Chaves Barreto Cristine,
Cardoso Alves Luciano,
Lima Aragão Francisco José,
Rech Elıćbio,
Schrank Augusto,
Henning Vainstein Marilene
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12711.x
Subject(s) - paecilomyces , conidium , entomopathogenic fungus , transformation (genetics) , biology , selectable marker , protoplast , fungus , botany , fungi imperfecti , microbiology and biotechnology , biological pest control , gene , genetics , beauveria bassiana
Two different methods, (i) PEG and (ii) biolistic, were employed to transform protoplasts and conidia of Paecilomyces fumosoroseus using hygromycin resistance as selectable marker. Transformation frequencies varied from 1.9 to 2.5 transformants μg −1 of DNA by the PEG method, and from 33 to 153 transformants μg −1 of DNA by the biolistic procedure.