Open AccessAmpicillin‐induced bacteriolysis of Escherichia coli is not affected by reduction in levels of anionic phospholipids
Author(s) -
Rodionov Dmitrii G,
Ishiguro Edward E
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12709.x
Subject(s) - phosphatidylglycerol , escherichia coli , cardiolipin , lysis , membrane , chemistry , bacteria , biochemistry , phospholipid , membrane protein , microbiology and biotechnology , biology , gene , genetics , phosphatidylcholine
Anionic phospholipids have been shown to interact with both membrane‐associated proteins and integral membrane proteins. The objective of this work was to determine whether bacteriolysis induced by treatment with ampicillin was influenced by the levels of anionic membrane phospholipids in Escherichia coli strain HDL11. The pgsA gene, encoding phosphatidylglycerophosphate synthase, in HDL11 is under the control of lacOP , and the levels of anionic membrane phospholipids are consequently dependent on IPTG. The results indicate that limiting the amounts of phosphatidylglycerol and cardiolipin did not affect the lysis process in both growing and nongrowing bacteria.