Open AccessDevelopment of mycobacterial species‐specific DNA probes by subtraction hybridization
Author(s) -
Hughes M.Siobhan,
Beck LesleyAnn,
Skuce Robin A.,
Neill Sydney D.
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12701.x
Subject(s) - biology , mycobacterium bovis , polymerase chain reaction , mycobacterium tuberculosis complex , genomic dna , mycobacterium , nucleic acid sequence , mycobacterium tuberculosis , dna , dna sequencing , hybridization probe , gene , microbiology and biotechnology , tuberculosis , genetics , bacteria , pathology , medicine
Subtraction hybridization was used to identify sequences of Mycobacterium bovis DNA which might be of diagnostic value. Genomic DNA from Mycobacterium avium , isolated commonly from cattle and whose tuberculin is used in the comparative intradermal tuberculin test, was subtracted from M. bovis genomic DNA. A novel sequence, of 131 bp, which appears to be Mycobacterium tuberculosis complex‐specific was identified. The specificity of this sequence was stringently tested by a probe and polymerase chain reaction (PCR) assay. Nucleotide identity determination and sequence comparisons revealed that the 131‐bp sequence is located directly upstream of a potential isocitrate dehydrogenase (IDH) coding gene and may be of diagnostic value, enabling differentiation of M. tuberculosis complex mycobacteria from other mycobacterial species.