Open AccessEnhancement of ColE1‐type replication promoted by the Gpα initiator protein of bacteriophage P4
Author(s) -
DıćazOrejas Ramón,
Lurz Rudi,
Rückert Beate
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12679.x
Subject(s) - replicon , cole1 , biology , replication factor c , dna replication , minichromosome maintenance , pre replication complex , origin of replication , origin recognition complex , microbiology and biotechnology , dna replication factor cdt1 , control of chromosome duplication , polymerase , eukaryotic dna replication , dna , genetics , plasmid
Gpα, the phage P4 specific replication protein, increases in vitro replication of pCN51, a pBR322 based replicon, by a factor of two. This effect is dependent on DNA polymerase I and requires transcription by host RNA polymerase. Electron microscopic analysis of replicating intermediates indicates that pCN51 replication occurred from the same origin and with the same directionality in the presence and in the absence of Gpα. These results reveal that Gpα can influence the replication of an heterologous replicon and show that this effect occurs in ColE1‐type replicons without altering the normal pattern of initiation. Further analysis of replicating intermediates shows an increase in the average size of the ColE1‐type replication ‘bubble’ obtained in the presence of Gpα. It is proposed that Gpα interacts with the ColE1 replisome complex at an early replication stage.