Open AccessCloning and analysis of methanol oxidation genes in the methylotroph Hyphomicrobium methylovorum GM2
Author(s) -
Tanaka Yasuhiro,
Yoshida Toyokazu,
Watanabe Katsumi,
Izumi Yoshikazu,
Mitsunaga Toshio
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12673.x
Subject(s) - methanol dehydrogenase , methylotroph , open reading frame , paracoccus denitrificans , biology , gene , genetics , microbiology and biotechnology , gene cluster , cytochrome , cloning (programming) , biochemistry , peptide sequence , bacteria , enzyme , computer science , programming language
The gene encoding the α‐subunit of methanol dehydrogenase ( mxaF ) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Paracoccus denitrificans and Methylophilus methylotrophus , respectively. The putative mxaF promoter sequence (−35 ‐AAAGACA‐, −10 ‐TAGAA‐) observed in other methylotrophs was not found in the region 5′ of H. methylovorum GM2 mxaF . Downstream of mxaF , five open reading frames and one partial open reading frame were detected which had high identity with the genes mxaJ , mxaG , mxaI , mxaR , mxaS and mxaA . This indicated the existence of a mxaFJGIRSA gene cluster in H. methylovorum GM2, as previously observed in M. extorquens AM1.