Open AccessCharacterization of Borrelia garinii isolated from Lyme disease patients in Hokkaido, Japan, by sequence analysis of OspA and OspB genes
Author(s) -
Wang Jianhui,
Masuzawa Toshiyuki,
Yanagihara Yasutake
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12669.x
Subject(s) - lyme disease , borrelia garinii , borrelia burgdorferi , biology , spirochaetaceae , microbiology and biotechnology , borrelia , sequence analysis , gene , virology , genetics , antibody
The outer surface proteins OspA and OspB genes of clinical Borrelia garinii isolates (JEM1–8) from Hokkaido, Japan were sequenced. One strain, JEM4, has a single ospA gene which is similar to European B. garinii strains PBr (sequence homology value: 94.1%) and T25 (91.2%). Five of the other seven strains exhibit a homologous C‐terminus (300 bp) on both ospA and ospB genes (88.7–97.3%). The other two strains seem to be derived from the five strains by ospA or ospB alterations. In a phylogenetic analysis based on ospA , these strains could be classified into B. garinii , but formed independent branches and separated from the typical B. garinii isolates from Europe and Russia.