Open AccessCharacterization of membrane surface proteins of Mycoplasma agalactiae during natural infection
Author(s) -
Tola Sebastiana,
Manunta Daniela,
Cocco Monica,
Turrini Franco,
Rocchigiani Angela M.,
Idini Graziano,
Angioi Antonio,
Leori Guido
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12667.x
Subject(s) - biology , antigen , membrane protein , antibody , mycoplasma , trypsin , microbiology and biotechnology , antigenic variation , mycoplasmataceae , immunoprecipitation , chemistry , mollicutes , biochemistry , membrane , enzyme , immunology
We have analyzed antigenic variation of seven M. agalactiae wild strains using different sera from naturally infected sheep. Only 30 day sera recognized all surface proteins and inhibited the growth of mycoplasmas. Furthermore, we have observed that two strongly immunogenic proteins: 55 and 35 kDa were digested using 500 μg/ml of trypsin. These two bands are immunoprecipitated together with four other proteins but only the 35 kDa protein is recognized by eluted antibodies.