Open AccessImportance of the galE gene on the virulence of Pasteurella multocida
Author(s) -
Fernández de Henestrosa Antonio R,
Badiola Ignacio,
Saco Montserrat,
Perez de Rozas Ana M,
Campoy Susana,
Barbé Jordi
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12661.x
Subject(s) - pasteurella multocida , mutant , gene , haemophilus influenzae , biology , virulence , microbiology and biotechnology , plasmid , genetics , nucleic acid sequence , locus (genetics) , salmonella , structural gene , peptide sequence , bacteria
The galE gene of Pasteurella multocida has been isolated by complementing galE ‐defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae . However, the gene organization surrounding the galE locus was different from that of H. influenzae . A galE ‐defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.