Open AccessThe regulation of Streptococcus mutans glucan‐binding protein A expression
Author(s) -
Banas Jeffrey A,
Potvin Heather C,
Singh Renita N
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12658.x
Subject(s) - streptococcus mutans , chloramphenicol acetyltransferase , sucrose , chloramphenicol , microbiology and biotechnology , gene expression , protein expression , biology , glucan , chemistry , gene , colonization , reporter gene , biochemistry , bacteria , antibiotics , genetics
The S. mutans GBP‐A is hypothesized to be constitutively expressed and to contribute to the sucrose‐dependent colonization of S. mutans . To investigate GBP‐A expression, a reporter gene encoding chloramphenicol acetyltransferase ( CAT ) was placed downstream of the gbpA promoter and CAT activity was measured under conditions that would be associated with the sucrose‐dependent colonization of S. mutans . Expression of GBP‐A was optimal under anaerobiosis and neutral pH conditions, and correlated with optimal growth. The addition of sucrose to the growth medium did not elevate the expression of GBP‐A.