Open AccessAn Escherichia coli gene divergently transcribed from a promoter overlapping the metA promoter
Author(s) -
Gery Sigal,
Ron Eliora Z
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12647.x
Subject(s) - escherichia coli , promoter , gene , biology , mutant , genetics , lac operon , translation (biology) , eukaryotic translation , fusion gene , microbiology and biotechnology , gene expression , messenger rna
The upstream region of the metA gene in Escherichia coli contains two promoters. We have identified by lacZ fusion an additional promoter in this region, and showed that it is transcribed in the opposite orientation from the metA gene. The putative translation product corresponds to a peptide of 147 amino acids – ORF19 by molecular mass. This peptide is probably not essential for growth, as an insertion mutant is viable.