Open AccessStability and modulated expression of a hygromycin resistance gene integrated in Botrytis cinerea transformants
Author(s) -
Hamada Walid,
Soulié MarieChristine,
Malfatti Pierrette,
Bompeix Gilbert,
Boccara Martine
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12642.x
Subject(s) - hygromycin b , biology , plasmid , botrytis cinerea , transformation (genetics) , conidiation , gene , heterokaryon , phenotype , genetics , protoplast , microbiology and biotechnology , mutant , botany
When transformation of Botrytis cinerea occurred in mononucleated protoplasts the hygromycin resistance phenotype was stable and integrated plasmid DNA although rearranged was transmitted through meiosis. We observed that transformants were often heterokaryotic and using serial conidial transfer, we showed failure of expression of the entire copies of integrated plasmids in some conidial isolates. A non‐Mendelian segregation of the hygromycin resistance phenotype was observed in most crosses between these transformants and sensitive strains. However, a 1:1 segregation ratio of plasmid DNA hybridisation was observed. Mechanisms of gene silencing in B. cinerea , in both the asexual and the sexual cycle, are discussed.