Open AccessInsertional inactivation of a Tet(K)/Tet(L) like transporter does not eliminate tetracycline resistance in Bacillus cereus
Author(s) -
Økstad Ole Andreas,
Grønstad Anne,
Lindbäck Toril,
Kolstø AnneBrit
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12641.x
Subject(s) - tetracycline , major facilitator superfamily , bacillus cereus , efflux , microbiology and biotechnology , mutant , tetr , biology , chemistry , gene , bacteria , biochemistry , genetics , antibiotics , gene expression , repressor
Bacillus cereus ATCC 10987 and ATCC 14579 can be induced to high levels of resistance to tetracycline. The chromosomal B. cereus gene bctl encodes a transmembrane protein with homology to Gram‐positive tetracycline efflux proteins and relation to other members of the major facilitator superfamily of transport proteins. A mutant strain containing an insertionally inactivated bctl gene did not show impaired tetracycline resistance. No additional altered phenotype was observed in the mutant. Accumulation studies suggested that the resistance mechanism involves a reduced sensitivity to intracellular tetracycline.