Open AccessConservation of cholera toxin gene in a strain of cholera toxin non‐producing Vibrio cholerae O1
Author(s) -
Honma Yasuko,
Iwanaga Masaaki
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12631.x
Subject(s) - vibrio cholerae , cholera toxin , pilus , microbiology and biotechnology , toxin , vibrionaceae , biology , cholera , el tor , blot , plasmid , virulence , gene , virology , bacteria , genetics
BT23, a Vibrio cholerae O1 El Tor isolate, possesses the cholera toxin (CT) gene as determined by PCR. However, CT was not detected in the culture medium by the reversed passive latex agglutination test, nor in the whole cell lysate as examined by Western blotting. The toxin‐coregulated pilus (TCP) was not detected by Western blotting. This suggests the presence of defects in the regulatory cascade. toxR , toxS and toxT , members of the regulatory cascade, were examined by PCR. toxR and toxS were conserved but toxT was not. CT and TCP production was complemented by transformation of toxT . The lack of toxT was suspected to be the cause of the undetectable production of CT in strain BT23.