Open AccessThe lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 expresses the monofucosyl A type 1 histo‐blood group epitope
Author(s) -
Monteiro Mario A,
Zheng P.Y,
Appelmelk Ben J,
Perry Malcolm B
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12630.x
Subject(s) - epitope , strain (injury) , lipopolysaccharide , microbiology and biotechnology , type (biology) , biology , virology , chemistry , antibody , immunology , anatomy , ecology
The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 was obtained by phenol‐water extraction of bacterial cells. Structural investigations were made on the lipid A free saccharide moiety released from the lipopolysaccharide by mild acetic acid hydrolysis. Nuclear magnetic resonance, gas liquid chromatography‐mass spectrometry and fast atom bombardment‐mass spectrometry were employed in the characterization of products from chemical manipulations. A monoclonal antibody specific for blood group A reacted strongly with lipopolysaccharide of H. mustelae . Chemical and serological data showed that the outer core region of the lipopolysaccharide from H. mustelae ATCC 43772 expresses the monofucosyl A type 1, α‐ d ‐GalNAc‐(1→3)‐[α‐ l ‐Fuc‐(1→2]‐β‐ d ‐Gal‐(1→3)‐β‐ d ‐GlcNAc, blood group determinant, a mimic of animal cell surface glycolipids and glycoproteins.