Open AccessCharacterization of a novel endo‐levanase and its gene from Bacillus sp. L7
Author(s) -
Miasnikov Andrei N
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12619.x
Subject(s) - bacillus subtilis , homology (biology) , gene , bacillaceae , biology , bacillales , homologous chromosome , enzyme , genetics , bacteria , biochemistry , microbiology and biotechnology
A novel endo‐levanase producing bacterium belonging to the Bacillus family has been isolated from soil. The enzyme was characterized and found to have no exo‐β‐fructofuranosidase activity. The endo‐levanase gene was cloned and sequenced. Homology searches have shown that the C‐terminal domain of the enzyme is homologous to a number of known β‐fructofuranosidases, including exo‐levanase from Bacillus subtilis and yeast invertases. The N‐terminal region of the endo‐levanase which is homologous to the C‐terminal sequence of the B. subtilis levanase appears to be a levan‐binding domain.