Open AccessEvidence for the role of 2‐hydroxychromene‐2‐carboxylate isomerase in the degradation of anthracene by Sphingomonas yanoikuyae B1
Author(s) -
Kim Eungbin,
Zylstra Gerben J,
Freeman James P,
Heinze Thomas M,
Deck Joanna,
Cerniglia Carl E
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12613.x
Subject(s) - anthracene , chemistry , sphingomonas , high performance liquid chromatography , metabolite , chromatography , strain (injury) , degradation (telecommunications) , carboxylate , naphthalene , biochemistry , organic chemistry , biology , telecommunications , 16s ribosomal rna , anatomy , computer science , gene
Abstract Sphingomonas yanoikuyae B1 is extremely versatile in its catabolic ability. An insertional mutant strain, S. yanoikuyae EK504, which is unable to grow on naphthalene due to the loss of 2‐hydroxychromene‐2‐carboxylate isomerase activity, was utilized to investigate the role of this enzyme in the degradation of anthracene by S. yanoikuyae B1. Although EK504 is unable to grow on anthracene, this strain could transform anthracene to some extent. A metabolite in the degradation of anthracene by EK504 was isolated by high‐pressure liquid chromatography (HPLC) and was identified as 6,7‐benzocoumarin by UV‐visible, gas‐chromatographic, HPLC/mass‐spectrometric, and 1 H nuclear magnetic resonance spectral techniques. The identification of 6,7‐benzocoumarin provides direct chemical and genetic evidence for the involvement of nahD in the degradation of anthracene by S. yanoikuyae B1.