Open AccessThe NAD synthetase NadE (OutB) of Bacillus subtilis is a σ B ‐dependent general stress protein
Author(s) -
Antelmann Haike,
Schmid Roland,
Hecker Michael
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12603.x
Subject(s) - sigma factor , bacillus subtilis , biology , promoter , nad+ kinase , microbiology and biotechnology , biochemistry , enzyme , genetics , gene , gene expression , bacteria
The identification of σ B ‐dependent general stress proteins is a useful strategy to understand the physiological role of the unspecific stress response in Bacillus subtilis . By N‐terminal sequencing of B. subtilis stress proteins Gsp38 was identified as the NAD‐synthetase (NadE). NadE was previously characterized as spore outgrowth factor B (OutB) conferring a temperature‐sensitive spore outgrowth defective phenotype. Transcriptional studies showed that nadE is strongly induced in response to heat, ethanol and salt stress or after starvation for glucose in a σ B ‐dependent manner. Two promoters are involved in transcriptional initiation, the σ A ‐dependent upstream promoter contributes to the basal level during growth, whereas the σ B ‐dependent downstream promoter is induced after different stress conditions.