Open AccessA thermal denaturation study of macronuclear chromatin in Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida)
Author(s) -
Salvini Mariangela,
Dalle Lucche Tiziana,
Durante Mauro
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12594.x
Subject(s) - tetrahymena , chromatin , ciliate , biology , tetrahymena pyriformis , macronucleus , protozoa , dna , microbiology and biotechnology , denaturation (fissile materials) , histone , biophysics , genetics , chemistry , nuclear chemistry
The macronuclear chromatin of the ciliate Blepharisma japonicum , in two starvation states, was studied by thermal denaturation analysis. The behaviour of B . japonicum chromatin, native and reconstituted with Tetrahymena pyriformis H1 histone, was analysed. The data obtained are consistent with the hypothesis that B. japonicum macronuclear chromatin contains a H1‐like peptide associated with the linker DNA, although this peptide is reduced in amount and/or chromatin stabilising ability when compared to Tetrahymena macronuclear H1.