Open AccessSelection and partial characterization of a Pseudomonas aeruginosa mono‐rhamnolipid deficient mutant
Author(s) -
Wild Marina,
Caro Alma Delia,
Hernández Ana Lilia,
Miller Raina M,
SoberónChávez Gloria
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12586.x
Subject(s) - rhamnolipid , pseudomonas aeruginosa , mutant , selection (genetic algorithm) , pseudomonadales , microbiology and biotechnology , pseudomonadaceae , chemistry , biology , bacteria , genetics , gene , computer science , artificial intelligence
Pseudomonas aeruginosa produces rhamnolipids which are tenso‐active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl‐β‐hydroxydecanoyl‐β‐hydroxydecanoate (mono‐rhamnolipid) and rhamnosyl‐rhamnosyl‐β‐hydroxydecanoyl‐β‐hydroxydecanoate (di‐rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono‐rhamnolipid while still producing di‐rhamnolipid. IBT8 was selected after random mutagenesis with Tn 501 ; yet, its mono‐rhamnolipid deficiency was found associated neither with its Tn 501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl‐transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.