Open AccessIdentification of Pediococcus acidilactici and Pediococcus pentosaceus based on 16S rRNA and ldhD gene‐targeted multiplex PCR analysis
Author(s) -
Mora Diego,
Fortina Maria Grazia,
Parini Carlo,
Manachini Pier Luigi
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12575.x
Subject(s) - pediococcus acidilactici , pediococcus , 16s ribosomal rna , microbiology and biotechnology , biology , ribosomal rna , gene , bacteria , genetics , lactobacillus , lactic acid , lactobacillus plantarum
A multiplex PCR assay that can readily and unambiguously identify Pediococcus acidilactici and Pediococcus pentosaceus strains was developed to give an easy‐to‐read profile based on the amplification of a 16S rRNA gene fragment, specific for each species, and a d ‐lactate dehydrogenase gene fragment specific for Pediococcus acidilactici strains.