Open AccessStress proteins and stress tolerance in an Antarctic, psychrophilic yeast, Candida psychrophila
Author(s) -
Deegenaars Michelle L,
Watson Kenneth
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12569.x
Subject(s) - psychrophile , heat shock protein , yeast , hsp60 , hydrogen peroxide , biology , mesophile , shock (circulatory) , heat shock , saccharomyces cerevisiae , microbiology and biotechnology , biochemistry , enzyme , hsp70 , bacteria , gene , genetics , medicine
Conditions are described for the heat shock acquisition of thermotolerance, peroxide tolerance and synthesis of heat shock proteins (hsps) in the Antarctic, psychrophilic yeast Candida psychrophila. Cells grown at 15°C and heat shocked at 25°C (3 h) acquired tolerance to heat (35°C) and hydrogen peroxide (100 mM). Novel heat shock inducible proteins at 80 and 110 kDa were observed as well as the presence of hsp 90, 70 and 60. The latter hsps were not significantly heat shock inducible. The absence of hsp 104 was intriguing and it was speculated that the 110 kDa protein may play a role in stress tolerance in psychrophilic yeasts, similar to that of hsp 104 in mesophilic species.