Open AccessRestriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel
Author(s) -
Eldar Avi,
Lawhon Sara,
F. Frelier Paul,
Assenta Liliana,
Simpson Bruce R,
Varner Patricia W,
Bercovier Herve
Publication year - 1997
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1997.tb12564.x
Subject(s) - streptococcus iniae , ribotyping , biology , restriction fragment length polymorphism , microbiology and biotechnology , 16s ribosomal rna , ecori , hindiii , ribosomal dna , tilapia , trout , vibrionaceae , restriction enzyme , genetics , genotype , bacteria , gene , fishery , phylogenetics , fish <actinopterygii>
Streptococcus iniae (junior synonym S. shiloi ) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. Hin dIII and Eco RI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178 T (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.